Journal: Molecular Therapy. Methods & Clinical Development

Article Title: Transduction Patterns of Adeno-associated Viral Vectors in a Laser-Induced Choroidal Neovascularization Mouse Model

doi: 10.1016/j.omtm.2018.01.008

Figure Lengend Snippet: Fluorescence Images of Control and AAV2-, AAV5-, and AAV-8-Injected Eyes Whole mounts of control eyes with (E) or without (A) choroidal neovascularization (CNV) showed only faint autofluorescence around the optic nerve head. In the absence of CNV, eyes injected with AAV2 (B) demonstrated diffuse, scattered fluorescence spots throughout the fundus, while eyes injected with AAV5 (C) or AAV8 (D) showed less fluorescence. In eyes with CNV, transduction of AAV of all three serotypes—AAV2 (F), -5 (G), and -8 (H)—was markedly increased around CNV lesions. The increase in transduction of AAV2 was greater than that of the other two serotypes. (I) The relative fluorescence intensities were as follows: AAV2/CNV − (n = 5), 2.45 ± 0.24; AAV2/CNV + (n = 10), 3.93 ± 0.32; AAV5/CNV − (n = 5), 1.88 ± 0.17; AAV5/CNV + (n = 10), 3.20 ± 0.19; AAV8/CNV − (n = 5), 2.07 ± 0.21; AAV8/CNV + (n = 10), 3.43 ± 0.21 (I). In the absence of CNV, there were significant differences in fluorescence intensity between control eyes and eyes injected with AAV (AAV2/CNV − , AAV5/CNV − , and AAV8/CNV − ) (p < 0.05). The fluorescence intensity differed significantly between eyes with and without CNV injected with all of the three AAV serotypes (AAV2, p < 0.017; AAV5, p < 0.024; AAV8, p < 0.021). Data are expressed as mean ± SEM. Significant differences: †p < 0.05 versus control eyes, ‡p < 0.05 versus eyes without CNV. Asterisk (*), CNV lesion. Scale bars, 200 μm.

Article Snippet: One microliter of AAV2-EGFP, AAV5-EGFP, and AAV8-EGFP (1 × 10 13 viral particles [vp]/ml) supplied by CdmoGen (Cheongju, Republic of Korea) were used for injection.

Techniques: Fluorescence, Injection, Transduction

Journal: Molecular Therapy. Methods & Clinical Development

Article Title: Transduction Patterns of Adeno-associated Viral Vectors in a Laser-Induced Choroidal Neovascularization Mouse Model

doi: 10.1016/j.omtm.2018.01.008

Figure Lengend Snippet: Colocalization of EGFP with CD11b, F4/80, and CD31 in Eyes Injected with AAV5 and AAV8 AAV5 (arrowheads in A, A-1, B, and B-1) and AAV8 (arrowheads in D, D-1, E, and E-1) transduction was observed in CD11b − and F4/80 + cells in the subretinal space and the INL, but not in these cells in CNV lesions. A small proportion of CD31 + endothelial cells was also positive for EGFP (C, C-1, F, and F-1). Asterisk (*), CNV lesion. (A–F) Scale bars, 100 μm; and (A-1–F-1) scale bars, 50 μm.

Article Snippet: One microliter of AAV2-EGFP, AAV5-EGFP, and AAV8-EGFP (1 × 10 13 viral particles [vp]/ml) supplied by CdmoGen (Cheongju, Republic of Korea) were used for injection.

Techniques: Injection, Transduction